【摘要】 目的探讨腺苷A1受体在基本呼吸节律产生和调节中的可能作用。方法制作新生大鼠离体延髓脑片标本,主要包含面神经后核内侧区(the medialregion of the nucleus retrofacialis,mNRF),并保留舌下神经根的完整,以改良Kreb’s液灌流脑片,稳定记录舌下神经根呼吸节律性放电(respiratory rhythmical discharge activity, RRDA)。在灌流液中先分别单独给予腺苷A1受体的特异性拮抗剂8-环戊-1,3-二丙基黄嘌呤(8-cyclopentyl-1,3-dipropylxa nthine, DPCPX)和特异性激动剂R-苯异丙基-腺苷(R-phenylisoprpyl-adeno sine,R-PIA);再分别先后给予R-PIA和R-PIA+ DPCPX,观察RRDA的变化,进一步探讨腺苷A1受体对其的调节作用。结果给予腺苷A1受体拮抗剂DPCPX后,呼气时程和呼吸周期明显缩短,吸气时程和积分幅度未出现显著性变化;给予腺苷A1受体激动剂R-PIA后,吸气时程,积分幅度显著性降低,呼吸周期和呼气时程明显延长,且R-PIA的呼吸抑制作用可部分被DPCPX逆转。结论腺苷A1受体参于了哺乳动物基本呼吸节律的产生和调节中起着重要的作用。更多还原

【Abstract】 Objective To explore the role of adenosine A1-receptors in the generation and regulation of central respiratory rhythm. Methods Neonatal rat brainstem slices including the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets retained were prepared in which the respiratory activity was recorded from the hypoglossal nerve rootlets by suction electrode. The possible role of adenosine A1-receptors on rhythmical respiration was investigated by administration of specific antagonist of adenosine A1-receptor R-phenylisopropyl-adenosine (R-PIA) and its antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) in modified Kreb’s solution (MKS) for perfusion of the brainstem slices. Results DPCPX induced a significant decrease in the expiratory time and respiratory cycles. However, R-PIA significantly prolonged the respiratory cycle and decreased the inspiratory time and the integral amplitude. The effect of R-PIA on the rhythmical discharges could be partially reversed by additional application of DPCPX. Conclusion Adenosine A1-receptors are involved in the modulation of rhythmical respiratory in neonate rat brainstem.更多还原