【摘要】 根据果蝇ebony基因的序列设计了一系列特异性引物,对1991年发现的一种黑腹果蝇的新的体色突 变(Qian&Zhang,1994)——黑条体突变型的ebony基因进行克隆和序列测定。与野生型W91910的ebony基因 相比,黑条体突变型的ebony基因的编码区无明显的大片段变异,变异仅存在于数个氨基酸位点,且均不位于 该基因产物的关键序列。在黑条体的ebony基因的5’端序列的克隆和序列测定中发现,与野生型W91910及黑檀体 e’的ebony基因相比,黑条体突变型的ebony基因有一个大片段的缺失,该缺失包括外显子l的206个碱基和 内含子1的747个碱基,由此确定了黑条体突变体的突变类型和突变位点。更多还原

【Abstract】 In this study we designed several pairs of primers to clone and sequence the ebony gene of a new pigment mutant named black strip (bsr) in Drosophila melanogaster (Qian & Zhang, 1994). Compared with ebony gene of wild type w91910 and ebony mutant of e1, there was no big deletion segment found in coding sequence of ebony of bsr except for several amino acid mutation sites which did not distribute in the key motif of ebony protein. However, a big fragment deletion was found at the 5’ end of ebony gene in bsr, which comprised 206 nucleotides of exon 1 and 747 nucleotides of intron 1 . Therefore we have been able to accurately localize the mutation locus in bsr.更多还原