【摘要】 采用生物测定和微量滴度酶标板法监测了桃蚜对高效氯氰菊酯抗性的发展趋势。结果表明 ,随着筛选次数增加 ,桃蚜对高效氯氰菊酯的抗药性逐渐增强 :筛选 3代后相对抗性倍数为 13 0 0 ,10代后为 4 9 86倍。随着抗性增强 ,桃蚜酯酶酶源蛋白含量从 35 86 32增加到 6 6 2 990 μg/mL ,酯酶活性从 33 6 76 0增加到 171 0 0 5 2μmol/ (mL·min)。比较抗性发展过程中酯酶酶源蛋白含量及其活性个体频率分布结果发现 ,随着选育次数的增多 ,更多的桃蚜个体分布在酯酶酶源蛋白含量和酯酶活性高的区域。相关分析结果表明 ,抗性筛选过程中的LD50 与酯酶含量及活性变化之间呈显著正相关 ,酯酶在桃蚜对高效氯氰菊酯抗药性发展中可能起着十分重要的作用。更多还原

【Abstract】 The resistance of alphamethrin in Myzus persicae (green peach aphid) was selected in laboratory. The resistance development was monitored through the bioassay and the 96-well microplate reader for measuring change of esterase (Est). The results showed that the resistance level gradually rose with the increase of selection time. After three generations, the resistance of green peach aphid was 13-fold. While ten generations later, the resistance amounted to 49.86-fold. It suggested that green peach aphid developed obvious resistance to alphamethrin. As resistance developed, enzyme protein content increased significantly from 35.8632 to 66.2990 μg/mL with Est activity varying from 33.6760 to 171.0052 μmol/(mL·min). Comparing the frequency distribution of enzyme protein content and activity, we found that more and more individuals possessed higher content and activity. And there is a significant correlation between LD 50 and enzyme protein content, Est activity respectively. The results showed that the Est played an important role in resistance development of green peach aphid to alphamethrin.更多还原