【摘要】 目的:阐述SARS患者病程与血清中SARS病毒含量间的关系.方法:采用高灵敏度荧光实时定量RT-PCR方法,对156份SARS患者进行检测,同时,以9.4×109cop ies/L(RobertKoch Institute)灭活的SARS冠状病毒为定量标准品,病毒样颗粒为阳性对照,分析SARS-CoV阳性血清样本中的病毒含量.结果:检测试剂的灵敏度达1×105cop ise/L,与相关病原体没有交叉.81份血清样本中病毒含量分布在1×105~1×107cop ies/L之间,患者血清样本中病毒含量较低,经统计学分析,病毒含量与病程长短无明显数量关系.结论:SARS患者血清中病毒含量低,与病程没有明显的相关性.更多还原

【Abstract】 AIM: To detect SARS-CoV in various clinical specimens such as sputum,gargling liquid,throat swab and stool and explore the association between the disease course and the SARS-CoV content.METHODS: We quantitatively detected 156 serum specimens from SARS patients by real time RT-PCR.The viral load in SARS-CoV positive serum was analyzed using the coronavirus deactivated with 9.4×10~9 copies/L.Robert Koch Institute as standard preparation and virus-like particles as positive controls.(RESULTS:) The sensitivity of the detected agent was 1×10~5 copies/L.Eighty-one of the 156 cases(51.9%) tested were positive with the viral load ranging from 1×10~5-(1×10~7) copies/L.The viral load in serum samples was generally low as compared with data reported for other specimens.CONCLUSION: There is no significant relationship between the low viral load and the disease course.更多还原