【摘要】 目的研究5型腺病毒载体(Ad5)携带外源p16基因对人脑恶性胶质瘤细胞系TJ899和TJ905生长状态的影响。方法免疫组化(S-P法)测定P16蛋白表达,甲基噻唑基四唑(MTT)法和克隆形成实验测定恶性脑胶质瘤细胞系生长状态。结果重组体腺病毒能介导p16外源基因在恶性脑胶质瘤细胞系TJ899和TJ905细胞中呈阳性表达,6 d时肿瘤细胞生长抑制率分别可达93.60%和98.28%。并且能显著地抑制肿瘤细胞的克隆形成能力。结论腺病毒介导p16基因能在肿瘤细胞中表达,并能明显抑制不同人脑恶性胶质瘤细胞系的生长状态。更多还原

【Abstract】 Objective To study the effect of the adenoviral vector(Ad5)expressing p16 gene on the growth of human glioma cell lines TJ899 and TJ905.Methods The DNA of adenovirus was extracted and amplified,then innoculated into 293 cells.Ad-p16 was transfected into TJ899 and TJ905 cells at multiplicity of infection(MOI).The expression of p16 was detected in TJ899 and TJ905 cells by immunohistochemical method.The proliferation of TJ899 and TJ905 cells was analyzed by methly thiazolyl tetrazolium(MTT)and colony formation.Results The p16 expression was observed in TJ899 and TJ905 cells.The growth rate of the Ad-p16 infected TJ899 and TJ905 cells was inhibited by 93% and 98.3%,as compared with that of the adenovirus with β-lactase gene(Ad-LacZ) infected cells.Colony formation in the Ad-p16 infected cells remarkably decreased as compared with Ad-LacZ infected cells.Conclusion The exogenous p16 mediated by adenovirus can inhibit the growth and colony formation of glioma cells.更多还原