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普通小麦抗、感赤霉病品种(系)的RAPD标记分析
RAPD Marker Analysis of Scab-resistant and -Susceptible Varieties (Lines) of Common Wheat
【摘要】 采用RAPD技术,用524个随机引物对3个抗病品种和4个感病品种的基因组DNA进行扩增,发现3个引物能在抗、感品种间检测到稳定的多态性。这3个引物及它们产生的多态片段是S3471220bp、S372875bp和S3751360bp,其中S3471220bp和S372875bp在感病品种上呈显性扩增,在抗病品种上无扩增,相反S3751360bp在感病品种上无扩增,在抗病品种上呈显性扩增。初步判断这3个特异带与小麦赤霉病抗性有关。
【Abstract】 524 random primers were used in RAPD analysis to amplify genomes of three scab-resistant varieties and four scab-susceptible varieties and three of them were found to show stable polymorphism in both the scab-resistant and -susceptible varieties.The polymorphic bands amplified with the three primers were S3471 220 bp,S372875 bp and S3751 360 bp.Of the bands,S3471 220 bp and S372875 bp were found in all the susceptible varieties but not in all the resistant varieties whereas S3751 360 bp was not found in all the susceptible varieties but found in all the resistant varieties. It was preliminarily concluded that these three specific bands related to scab resistance.
- 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2005年05期
- 【分类号】S512.1
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