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辣(甜)椒种质资源的RAPD分析(英文)

RAPD Analysis of Chili (Pimiento) (Capsicum annuum) Germplasms

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【作者】 孙凌云盖树鹏樊治成孟祥栋

【Author】 SUN Ling-yun~1,GAI Shu-peng~2,FAN Zhi-cheng~2,MENG Xiang-dong~2(1 Department of Biochemistry,Taishan Medical College,Taian,Shandong 271000,China;2 Department of Horticulture,Shandong Agricultural University,Taian,Shandong 271018,China)

【机构】 泰山医学院生物化学教研室山东农业大学园艺学院山东农业大学园艺学院 山东泰安271000山东泰安271018山东泰安271018

【摘要】 用RAPD技术对我国的34个辣(甜)椒品种进行了分析,22个随机引物共扩增出119条带,其中67条具多态性。采用Nei、Jaccard和欧氏距离3种方法计算各品种间的遗传距离矩阵,分别根据3个遗传距离矩阵进行UPGMA聚类分析,得出3个树形聚类图。聚类结果表明,IBPGR将辣椒种划分为4个变种的建议更为合理,Nei方法与Jaccard方法的结果均认为圆锥椒与灯笼椒的亲缘关系较近,而欧氏距离法的结果则认为所有辛辣类型的亲缘关系较近。研究还发现,栽培及育种活动在变种的发展过程中起到了重要作用。

【Abstract】 RAPD analysis was employed to analyze 34 chili (pimiento) varieties and its 22 random primers amplified 119 bands of which six seven were polymorphic.Nei’s method,Jaccard’s method,and Euclidean distance method were adopted to calculate the matrixes of the genetic distances of various varieties and then three tree diagrams were constructed in accordance with the matrixes in UPGMA cluster analysis.The cluster analysis revealed that IBPGR’s chili classification that Capsicum annuum could be divided into four varieties was relatively reasonable compared with those by Nei’s method and Jaccard’s method that var.conoides had close genetic relation with var.grossum,and that by Euclidean distance method that all chili types had close genetic relations.In addition,it was found that farming practices and breeding had played an important role in the developments of these varieties.

【关键词】 RAPD辣(甜)椒聚类分析
【Key words】 RAPDchili (pimento)cluster analysis
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2005年05期
  • 【分类号】S641.3
  • 【被引频次】13
  • 【下载频次】135
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