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铝胁迫下小麦根部苹果酸和柠檬酸的直接测定

Direct Determination of Malic and Citric Acids in Wheat Roots under Aluminum Stress

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【作者】 杨若明; 张经华;

【Author】 YANG Ruo ming 1, ZHANG Jing hua 2 (1 College of Life and Environmental Sciences, The Central University for Nationalities. Beijing 100081,China;2 Faculty of Environmental Studies, Nagasaki University,Nagasaki 8528521,Japan)

【机构】 中央民族大学生命与环境科学学院; 长崎大学环境科学部 北京; 100081; 日本长崎; 8528521;

【摘要】 铝的有害性严重制约了约占世界可耕地面积40%的酸性土壤中的作物生产.从植物的培养液中发现从根部分泌出的有机酸与铝结合从而实现无毒化是其抗铝逆性机理的重要依据.而本文直接测定了铝胁迫下培育的小麦根中的铝和有机酸含量,确认了主要积累的是苹果酸和柠檬酸.发现随着提高培养液中的铝浓度,根部的铝含量也相应增加.同时,虽然根中的柠檬酸含量无明显变化,但苹果酸被诱导增加.通过对有机酸与铝的络合能力的调查,探讨了对植物抗铝逆性强弱的影响.

【Abstract】 Aluminum toxicity limits crop prodution on acidic land, which accounts for 40% arable land in the world.Organic acids secreted by plant roots were found capable of combining with Aluminum to realize Aluminum detoxification in plant culture solution and this was the basis for plant resistance mechanism to aluminum stress. The study directly determine organic acid and aluminum contents in wheat roots under aluminum stress thereby confirming that accumulated organic acids were mainly malic and citric acids. As aluminum concentration increased in the culture solution, aluminum content in the roots corresponding increased. Meanwhile, malic acid content was found to increase due to the external induction, although citric acid content did not remarkably varied in the roots. The complexing capabilities of organic acids to aluminum were determined to probe their effects on plant aluminum resistance.

【关键词】 小麦; 铝胁迫; 有机酸; 苹果酸; 柠檬酸;
【Key words】 wheat; aluminum stress; malic acid; citric acid;
【基金】 中央民族大学211项目资助
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2005年04期
  • 【分类号】S512.1
  • 【被引频次】5
  • 【下载频次】410
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