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不同发育期中华绒螯蟹胚胎离体孵化和幼体培育的研究
Effects of embryonic developmental stages in vitro from fertilized eggs in Chinese mitten crab Eriocheir sinensis on larva rearing
【摘要】 将中华绒螯蟹Eriocheir sinensis不同发育期的胚胎从腹肢基部剪下后在室温下孵化,结果表明,处于卵裂期、囊胚期和原肠期的胚胎离体孵化的孵化率(42.16%~54.90%)无显著差异(P>0.05);从原肠期开始,离体孵化的孵化率随着离体时间的缩短呈升高的趋势,即眼点期(68.95%±5.89%)和心跳期(77.47%±5.45%)极显著高于(P<0.01)前3个时期,对外界环境的适应性也增强,这5个时期离体胚胎孵出的溞状幼体的变态率差异不显著(P>0.05)。试验证明,剪掉抱卵腹肢进行胚胎离体孵化,不但促进了亲蟹卵巢的快速发育,而且还降低了投喂、调节水质等费用。
【Abstract】 Cleavage, blastula and gastrula were separated from matured Chinese mitten crab and incubated in vitro. The results showed that the hatching rate, varying from 42.16% to 54.90%, was no significant difference (P>0.05), even though there was an in increase in hatching rate with the embryonic development (eyed stage, 68.95%±5.89%; heart beating stage, 77.47%±5.45%). The metamorphosis of the larvae from the stages above was the same, indicating that this is feasible for efficient commercial larva production.
【Key words】 Eriocheir sinensis; fertilized egg; in vitro; larva; developmental stage; incubation;
- 【文献出处】 大连水产学院学报 ,Journal of Dalian Fisheries University , 编辑部邮箱 ,2005年03期
- 【分类号】S966.16
- 【被引频次】13
- 【下载频次】330