【摘要】 水解大豆皂甙分子上的部分糖基可以生成低糖链、高活性的新皂甙。实验步骤是:采用A.niger848s水解大豆皂甙,生成3组不同的新皂甙;并用1 mm厚的层析制备板分离提取各个新皂甙。结果表明最适当的展开剂V(氯仿)∶V(甲醇)∶V(水)=74∶26∶10。该酶解过程的有效性已通过薄层层析(TLC)和高效液相色谱(HPLC)分析证实。更多还原

【Abstract】 Enzymatic hydrolysis of soybean saponin sugar-moiety produces new saponins,containing lower sugar and higher activity.The experiment procure is follows: using A.niger848s to produce three new kinds of saponins;each new saponins is obtained by silica of 1mm.It reveals that the optimal developing solvent is chloroform,with methanol∶water=74∶26∶10.Effectiveness of the procedure is verified by TLC and HPLC analyses.更多还原