【摘要】 根据芦丁 α 鼠李糖苷酶N端氨基酸序列设计简并引物,从Absidiasp.R9g的总RNA出发,通过逆转录 聚合酶链反应(RT PCR)扩增出芦丁 α 鼠李糖苷酶基因片段。将RT PCR获得的1条1.6kb的目的条带与pMD18 T载体连接,转化大肠杆菌JM109感受态细胞。酶切鉴定结果表明目的片段克隆成功。更多还原

【Abstract】 RT-PCR was carried out with degenerate primers designed according to the N-terminal amino acid sequences of rutin-α-rhamnosidase. A 1.6?kb cDNA fragment was amplified from the total RNA of Absidia sp.R9g and ligated with vector pMD18-T.E.coli JM109 competent cells were transformed with the recombinant plasmid. The result shows that clones are successful.更多还原