【摘要】 目的:制备抗Annexin B1单克隆抗体,为进一步研究Annexin B1的生理功能奠定基础。方法:制备了具有抗原性的Annexin B1,并采用脾内包埋法免疫小鼠,无菌取出脾细胞与骨髓瘤细胞SP2/0进行融合,随后以ELISA方法和有限稀释法进行3次筛选和亚克隆。结果:用杂交瘤技术,我们获得了1株杂交瘤细胞株,能稳定地分泌高滴度的针对Annexin B1的特异单抗。结论:成功筛选并制备了Annexin B1的特异单抗,为下一步功能研究打下了良好的基础。更多还原

【Abstract】 Objective:To prepare monoclonal antibody against Annexin B for further studying the physiological function of Annexin B1.Methods: Annexin B1 was expressed and purified in E.coli and its antigenicity was confirmed.The product was used to immunize BALB/c mice by intrasplenic embedding.Spleen cells of BALB/c mice were obtained and fused with myeloma cell line SP2/0 in a HAT medium containing 50% PEG 4000 and were then subjected to incubation in 37℃,5% CO₂incubator for several days.ELISA was used to assay the type and titer of the McAbs in the supernatant.Results: A hybridoma cell line 2B10H5,which can steadily secrete specific McAbs,was obtained.Conclusion: The McAbs of Annexin B1 have been successfully prepared,which pave a way for further investigation of Annexin B1.更多还原