【摘要】 目的探讨白细胞介素-1β(IL-1β)对体外培养人牙周膜(PDL)细胞生物活性的影响。方法体外培养人PDL细胞,分别与0.1、0.5、1、5和10ng/ml的IL-1β作用3d,或在10ng/ml的IL-1β作用1、2、3、4、5d后,用噻唑盐(MTT)法检测PDL细胞增殖的情况;在10ng/ml的IL-1β作用4d后用ELISA法检测纤维连接蛋白(Fn)的含量,用酶动力学方法检测碱性磷酸酶(ALP)活性。结果IL-1β以浓度依赖的方式抑制PDL细胞的增殖,最小抑制浓度为1ng/nl(P<0.05)。在IL-1β作用下,PDL细胞合成Fn的水平明显下降(P<0.001),并强烈抑制PDL细胞的ALP活性(P<0.001)。结论IL-1β可能通过抑制PDL细胞的增殖以及抑制ALP活性和Fn的合成,影响牙周病的发生和修复。更多还原

【Abstract】 Objective To explore the effects of interleukin- 1β(IL-1β)on the biological activity of human periodontal ligament(PDL) cells in vitro. Methods Human PDL cells were cultured in DMEM medium containing IL-1β(0.1,0.5,1,5 and 10ng/ml) for 1,2,3,4 and 5 days, respectively. The proliferation of PDL cells was measured by MTT assay at the first, second, third, fourth and fifth days after IL-1β treatment, respectively. Fibronectin level in the medium was determined by ELISA at the fourth days after 10ng/ml IL-1β treatment, and alkaline phosphatase(ALP) activity was measured by enzyme kinetic method. Results IL-1β inhibited the growth of human PDL cells in a dose-dependent manner, and its lowest effective concentration was 1ng/ml(P<0.05). IL-1β singnificantly inhibited the fibronectin synthesis and ALP activity in human PDL cells(P<0.001). Conclusion IL-1β may play an important role in the pathogenesis and repair of periodontal diseases.更多还原