【摘要】 目的研究他莫昔芬(TAM)诱导不同生长状态下雌激素受体阳性(ER(+))乳腺癌细胞凋亡的周期特异性并比较其差异。方法体外培养的ER(+)乳腺癌细胞株MCF-7细胞株和原代培养的ER(+)乳腺癌细胞用TAM作用后,在流式细胞仪(FCM)上用亚G1峰(sub-G1)法检测细胞凋亡率,用细胞周期特异性细胞凋亡检测法(API)检测细胞凋亡的周期特异性。结果TAM诱导不同生长状态的ER(+)乳腺癌细胞凋亡均为G0/G1期发生的周期特异性细胞凋亡,但TAM在体外诱导的乳腺癌细胞凋亡率高于在体乳腺癌细胞。结论在ER(+)乳腺癌细胞中TAM在G0/G1期特异性诱导细胞凋亡,但在不同的生长状态下,TAM诱导细胞凋亡的效率不同。更多还原

【Abstract】 Objective To study the cell-cycle specificity of tamoxifen-induced apoptosis in the ER(+) breast cancer cells in vitro and in vivo. Methods ER(+) MCF-7 cell line (in vitro) and primary cultured ER(+) breast cancer cells (in vivo) were treated with tamoxifen, and the cell cycle specificity of cell apoptosis and the apoptotic rate were dertermined by flow cytometry. Results Both MCF-7 and primary cultured breast cancer cells were induced to apoptosis with G 0/G 1 phase specificity by tamoxifen. And the apoptotic rate in MCF-7 was higher than that in primary cultured breast cancer cells. Conclusion Tamoxifen could induced G 0/G 1 phase specific apoptosis in the ER(+) breast cnacer cells, and tamoxifen-induced apoptotic rate was higher in vitro than in vivo.更多还原