【摘要】 目的　研究蟾酥作用后HL -60细胞基因表达谱的变化 ,为蟾酥诱导HL -60细胞凋亡的可能机制提供依据。方法　采用细胞形态学观察、DNA琼脂糖凝胶电泳检测细胞凋亡 ;应用DNA芯片技术检测蟾酥作用前后HL -60细胞基因表达谱的变化。结果　 1% (v/v)的蟾酥可明显诱导HL -60细胞凋亡。细胞凋亡发生前 ,在检测的 115 2个基因中共有 9个基因表达改变 ,其中 8个基因上调 ,1个基因下调。结论　在蟾酥诱导HL -60细胞发生凋亡过程中有多个基因参与 ,BCL -2下调以及IL -8上调与凋亡的发生关系密切。更多还原

【Abstract】 Objective To study the changes of gene expression profiling in HL-60 cells treated by Chan’su, and the possible mechanisms of Chan’su-induced apoptosis in HL-60 cells. Methods Apoptosis was determined by morphology and agarose gel electrophoresis of DNA fragmentation. The changes of gene expression profiling were detected with DNA microarray. Results 1%(v/v) Chan’su could obviously induce HL-60 cells apoptosis. In the tested 1152 genes, mRNA expression levels of 9 genes changed prior to apoptosis in Chan’su-treated HL-60 cells. Of the 9 genes, 8 gene mRNA levels were up-regulated and 1 gene mRNA level was down-regulated. Conclusion Apoptosis in HL-60 cells treated by Chan’su was involved in many genes. Bcl-2 down-regulation and IL-8 up-regulation were closely correlated with the Chan’su-induced HL-60 cells apoptosis.更多还原