【摘要】 目的　通过观察结直肠腺癌中胰岛素样生长因子 -Ⅱ(IGF -Ⅱ)mRNA的表达 ,并结合肿瘤细胞增殖和凋亡状态的研究 ,探讨IGF -Ⅱ基因表达与细胞增殖和凋亡的关系 ,并分析其意义。方法　选取 60例结直肠腺癌石蜡标本 ,采用原位分子杂交法检测IGF -ⅡmRNA ,免疫组化S -P法检测PCNA蛋白 ,TUNEL法检测细胞凋亡 ,多媒体图文分析系统定量肿瘤阳性细胞率。以 15例正常结直肠组织作为对照。结果　结直肠腺癌中IGF -ⅡmRNA阳性细胞率 (3 9 64 %± 7 3 2 % )显著高于正常结直肠组织 (2 3 2 1%± 4 10 % )(P <0 0 1) ;IGF -ⅡmRNA的表达与肿瘤Dukes分期和淋巴结转移有关 ,而与患者的年龄、性别、肿瘤大小、部位和分化程度无关 ;IGF -ⅡmRNA的表达与PCNA、细胞凋亡均呈显著正相关。结论　IGF -Ⅱ可能通过促进细胞增殖和凋亡参与了肿瘤的进展。更多还原

【Abstract】 Objective To investigate the relationship among IGF-II expression and cell proliferation and apoptosis in human colorectal adenocarcinoma. Methods 60 paraffin embedded samples of human colorectal adenocarcinomas were used in this study. IGF-ⅡmRNA expression was detected by in situ hybridization, proliferation cell nuclear antigen(PCNA) protein was measured by immunohistochemical staining and apoptosis was determined by TUNEL technology. Fifteen normal colorectal tissues were used as controls. Computerized image analysis system qualitatively analyzed the positive ratio of above three indices in the cancer and normal tissues. Results The percentage of IGF-ⅡmRNA expression was significantly higher in colorectal adenocarcinoma (39.64%±7.32%) than that in normal colorectal tissues (23.21%±4.10%) (P<0.01), was related to Dukes stages and LN metastasis, and was not related to the patient age and gender,and tumor site, size and differentiation. There was a positive correlation among IGF-ⅡmRNA, PCNA expresion and cell apoptosis in colorectal adenocarcinomas(P<0.01). Conclusion IGF-Ⅱ may play a role in the pathogenesis of colorectal adenocarcinoma by promoting cell proliferation and inhibiting cell apoptosis.更多还原