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大豆胚芽中α-V_E含量的HPLC测定

HPL C DETERMINATION OF α-V_E IN SOYBEAN GERM

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【作者】 刘云朱丹华

【Author】 Liu Yun~(1,2) Zhu Danhua~1(1.National Soybean Improvement Sub-center,Zhejiang Academy of Agricultural Science,Hangzhou,310021;2.College of Food Science and Technology,ZhejiangUniversity,Hangzhou,310029)

【机构】 浙江省农业科学院杭州国家大豆改良分中心浙江省农业科学院杭州国家大豆改良分中心 杭州310021浙江大学食品学院杭州310029杭州310021

【摘要】 用反相高效液相色谱法测定了大豆胚芽中的α-VE含量,比较了两种不同前处理方法对样品中α-VE含量的影响。结果表明,大豆胚芽中α-VE含量为36.60~46.10mg/100g样品;样品预处理时,超声离心法比皂化离心法处理,测得样品中α-VE含量高出8.29%~15.39%;样品预处理过程中加入抗坏血酸比不加抗坏血酸,测得α-VE含量要高出2.21%~9.16%。

【Abstract】 The content of α-V_E in soybean germ determined by RF-HPLC the effect of two different pretreatment methods on the content of α-V_E in sample was studied.The results showed that the content of α-V_E in soybean germ was 36.60~46.10mg/100g germ.Sample was prepared through ultrasonic wave enhancing extraction,the content of α-V_E was 8.29%-15.39% higher than that through saponification extraction.The effect of L-Aseorbic acid on the experimental results of α-V_E was also obvious.

【关键词】 大豆胚芽α-VE测定高效液相色谱法
【Key words】 Soybean germVitamin EdeterminationHPLC
  • 【分类号】R151
  • 【被引频次】1
  • 【下载频次】102
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