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大豆胚芽中α-V_E含量的HPLC测定
HPL C DETERMINATION OF α-V_E IN SOYBEAN GERM
【摘要】 用反相高效液相色谱法测定了大豆胚芽中的α-VE含量,比较了两种不同前处理方法对样品中α-VE含量的影响。结果表明,大豆胚芽中α-VE含量为36.60~46.10mg/100g样品;样品预处理时,超声离心法比皂化离心法处理,测得样品中α-VE含量高出8.29%~15.39%;样品预处理过程中加入抗坏血酸比不加抗坏血酸,测得α-VE含量要高出2.21%~9.16%。
【Abstract】 The content of α-V_E in soybean germ determined by RF-HPLC the effect of two different pretreatment methods on the content of α-V_E in sample was studied.The results showed that the content of α-V_E in soybean germ was 36.60~46.10mg/100g germ.Sample was prepared through ultrasonic wave enhancing extraction,the content of α-V_E was 8.29%-15.39% higher than that through saponification extraction.The effect of L-Aseorbic acid on the experimental results of α-V_E was also obvious.
- 【文献出处】 大豆科学 ,Soybean Science , 编辑部邮箱 ,2005年04期
- 【分类号】R151
- 【被引频次】1
- 【下载频次】102