【摘要】 实验构建了CaMV35S启动子控制下的大豆抗病相关基因SR1的正反义植物双元表达载体pBISR1(+)和pBISR1(-)。通过根癌农杆菌叶盘转化法,将正义和反义SR1 基因导入烟草Ha vana 425,经卡那霉素筛选,获得了抗性植株。经PCR和PCR-Southern印迹分析,证明抗性植株中整和了SR1基因,RT-PCR分析进一步表明正义和反义基因皆能转录为完整的mRNA,经疫霉根腐接种及抗病性鉴定表明,转反义基因株系和未转基因株系均轻微感病,而转正义基因株系始终没有出现感病症状。更多还原

【Abstract】 Two plant expression vectors carrying sense or antisense soybean resistance-related gene SR1 under the regulation of cauliflower mosaic virus 35s promoter was constructed. Leaf segments of tobacco Havana 425 were infected by Agrobacterium tumefaciens LBA4404 with pBISR1(+) or pBISR1(-), from which kanamycin resistant plants were obtained. PCR and PCR-Southern analysis proved that the SR1 gene was integrated into the genomes of the tobacco plants, and RT-PCR analysis proved that sense or antisense gene was transcripted into a complete mRNA. The disease resistance assay showed that plants with antisense gene and control plants were slightly susceptible, and plants with sense gene were not susceptible.更多还原