【摘要】 以应县小黑豆的下胚轴、子叶、小真叶为试验材料,研究不同培养基对小黑豆愈伤组织诱导和器官分化的影响。结果表明:NAA和BA单独使用不利用小黑豆的离体培养,细跑分裂素与生长素结合使用有利于小黑豆愈伤组织的诱导和器官分化。下胚轴在 Ms + BA2mg/L + IAA0.5mg/L、Ms+BA1.5mg/L+IAA0.5mg/L Ms+KT2mg/L+NAA0.2mg/L培养基中植株再生频率为46%、46%、16%,在前两种培养基中下胚轴外植体分化出丛生芽。小黑豆下胚轴愈伤组织诱导率、再生植株分化率较子叶、小真叶高。更多还原

【Abstract】 The effect of variant culture medium on callus inducement and shoot differentiated was studied with first lamina,cotyledons,hypocotyls explants of Yingxian Xiaoheidou.The results indicated that BA and NAA had no advantages on tissue culture of Yingxian Xiaoheidou,but combinations of cytokinin and auxin had advantages on callus inducement and organ differentiation.Culture mediums of Ms+BA2mg/L+IAA0.5mg/LMs+BA1.5mg/L+IAA0.5mg/L and Ms+KT2mg/L+NAA0.2mg/L,had different rate of plant regeneration with 46%,46%,16% respectively,In the first and second culture medium had got multiple shoot。The rates of callus inducement and plant regeneration of soybean hypocotyls were higher than that of lamina and cotyledons.更多还原