【摘要】 根据人脐静脉血管内皮细胞(ECV-304)的显微和超微结构,研究了银杏叶多糖(GBLP)对ECV-304细胞和HL-60细胞粘附作用的影响.结果表明,正常生长的ECV-304细胞呈不规则形,细胞质丰富,细胞核呈圆形或椭圆形.电镜下,可见细胞表面有绒毛,细胞内具有Weibel-Palade小体.当ECV-304细胞受凝血酶(thrombin)刺激后,与HL-60细胞的粘附能力明显提高;而当用200μg/mL和500μg/mL剂量的GBLP处理ECV-304细胞后,与HL-60细胞的粘附率明显下降.这表明200μg/mL和500μg/mL剂量的GBLP对人脐静脉内皮细胞与HL-60细胞粘附有抑制作用.更多还原

【Abstract】 ECV-304 cells were observed under microscope and electron microscope,and the effects of GBLP on the adhesion of HL-60 cells to ECV-304 cells were studied.The results showed that ECV-304 cells are a kind of irregular cells under microscope.Under electron microscope,the microvilli on cell surface and Weibel-Palade bodies in cell could be observed.When ECV-304 cells were stimulated with thrombin,the adhesion ability of HL-60 cells to ECV-304 cells was enhanced.When ECV-304 cells were treated with GBLP at 200 μg/mL and 500 μg/mL concentration,the ratio of adhesion cells were decreased.Conclusion:GBLP of 200 μg/mL and 500 μg/mL dose can inhibit HL-60 cell adhesion to ECV-304 cell.更多还原