【摘要】 从我国名贵中药人参的根中提取出水溶性粗多糖.粗多糖经脱淀粉、冻融分级、SepharoseCL-4B柱层析分级和脱蛋白等方法,分离纯化出人参果胶SB.经DEAE-SephadexA-25柱层析等方法证明SB为均一组分.气相色谱分析表明,人参果胶SB的单糖组成为GalA,Gal,Ara和Rha,物质的量比依次为1.32∶1.00∶0.34∶0.15.人参果胶SB的梯度部分酸水解分析表明,GalA和Gal构成了SB的主链部分,其中GalA为主链的核心组分,Gal,Ara和Rha构成了主侧链结构的边缘及末端残基部分.以上结果说明,人参果胶SB是以半乳糖醛酸为主的酸性杂多糖.更多还原

【Abstract】 <Abstrcat>A water-soluble polysaccharide was isolated from the root of Panax ginseng C. A. Mey. The crude polysaccharide was fractionated and purified by deamylumized, freezed-melted, gel filtration on Sepharose CL-4B and deproteinized, so pectin SB was abstained. The analysis of gel filtration on DEAE-Sephadex A-25 indicated that SB was homogenous. The GC analysis indicated that SB contained the monosaccharides of GalA, Gal, Ara and Rha and their molar ratio is 1.32∶1.00∶0.34∶0.15.The gradient partial hydrolysis with acid was used for the structure analysis of SB. The results revealed that the main chain of SB is composed of GalA and Gal, the inner is GalA. The border of the main chain and the side chain and the end is composed of Gal, Ara and Rha. All results indicated that SB is an acidic heteropolysaccharide.更多还原