【摘要】 根据已报道的犬科狗的Gdf8基因cDNA编码序列设计一对引物,利用RT-PCR技术扩增银狐的Gdf8基因cDNA的编码序列,将PCR产物连接到质粒pMD18-T载体上,然后转化到JM109大肠杆菌中,得到阳性克隆,同时进行序列的测定和分析。测序结果与已报道的Gdf8基因cDNA编码序列的大小相同,为1128bp。该序列与狗的同源性为99.2%,仅有9个核苷酸差异,推断的氨基酸序列仅有3个差异。更多还原

【Abstract】 According to cDNA sequence of Gdf8 gene of dog, a pair of primers was designed b y the PrimerSelect software. With the primers, RT-PCR was performed and the cDNA fragment of Gdf8 gene in the silver fox was obtained. The fragment was cloned i nto the pMD18-T vector using TA clone method. The identified positive clone was sequenced. The result showed that the sequence comprised the complete coding se quence of Gdf8 gene and its length was 1128bp. Compared with dogs, 99.2% nuc leotides were homologous, and there were nine point mutations, which caused thre e mutations in the predicted amino acid sequences.更多还原