【摘要】 RNA干扰(RNA interference,RNAi)是通过短双链RNA(small interference RNA,siRNA)介导的特异性抑制相应基因表达的新技术。作为研究基因功能的有效手段,该技术已在线虫、果蝇、斑马鱼和鼠等动物中进行了应用。本研究利用体外转录合成的短双链干扰RNA(siRNA),针对鸡的管家基因3-磷酸甘油醛脱氢酶基因(glyceraldehyde-3-phosphate dehydrogenase,GAPDH),在鸡胚成纤维细胞中对其表达进行干扰。结果显示siGAPDH能有效阻断GAPDH基因在鸡胚成纤维细胞中的表达,而不相关的siRNA则对该基因的表达没有影响,证明在鸡细胞中也存在RNA干扰现象,为利用RNAi技术对鸡的基因功能研究奠定了一定基础。更多还原

【Abstract】 RNA interference (RNAi) is a new methodology to inhibit gene expression specifically by small interference RNA triggering. As an efficient approach, it has been widely used to study gene function on Caenorhabditis elegans. Drosophila, zebrafish and mouse. Here, we inhibit the expression of GAPDH gene in chicken embryonic fibroblast cells by used siRNA that was synthesized through transcription in vitro. The result shows that the siRNA can inhibit the expression of GAPDH gene efficiently but the siIRR can not. Its means there is RNAi mechanism in chicken embryonic fibroblast cells. This experiment will be helpful to the researching gene function of chicken by used RNA interference.更多还原