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牧草根际3种平脐蠕孢菌培养性状及对营养的利用能力

The culture characteristic and nutrition utilization of three species of Bipolaris

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【作者】 陈秀蓉; 南志标; 杨成德; 任继周; 李亚利;

【Author】 CHENG Xiu-rong~1,NAN Zhi-biao~2,YANG Cheng-de~1,REN Ji-zhou~1,LI Ya-li~1(1.Pratacultyral College of Gansu Agriculture University,Lanzhou 730070,China 2.College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 73000,China)

【机构】 甘肃农业大学草业学院; 兰州大学草地农业科技学院; 甘肃农业大学草业学院 甘肃兰州730070; 甘肃兰州730020; 甘肃兰州730070; 甘肃兰州730070;

【摘要】 首次对我国典型草原牧草根际特异平脐蠕孢菌、高粱平脐蠕孢菌及麦根腐平脐蠕孢菌3种真菌的培养特性进行了研究。结果表明,3种平脐蠕孢菌菌丝生长的温限均为10~35℃,最适温度30℃。在试验的11种碳源中,D-果糖等碳源对3种平脐蠕孢菌菌丝生长有明显的促进作用,菊糖、氯醛糖有较强的抑制作用。在试验的12种氮源中,亮氨酸、L-谷氨酸、硝酸铵有明显的促进作用,而L-精氨酸、尿素、碳酸铵、甘氨酸有较强的抑制作用。高粱平脐蠕孢菌对多种碳、氮源利用能力较强,麦根腐平脐蠕孢菌则较弱。3种平脐蠕孢菌在不同碳、氮源培养基上菌落颜色、大小、边缘整齐度有明显差异。

【Abstract】 This study focused on the characteristics of Bipolaris specifera,B.sorghicola and B.sorokiniana when grown in a culture.These species are from the grass rhizome of the steppe in eastern Gansu.The results showed hyphae from all three species could grow in the temperature range of 10-35℃,with the optimum hyphae growth occurring at 30℃.The hyphae grew fast on media with D-fructose as the C-source and either leucine,L-glutamic acid or ammonium nitrate as the N-source,but grew slowly on media with inulin or chloralose puriss as the C-source and either L-arginine,urea,ammonium carbonate or glycine as the N-source.B.sorghicola’s ability of to utilize the C-source and N-sources was the best of the three species,with B.sorokiniana the worst.There were evident differences in color,size and edge of clony between the Bipolaris species.

【关键词】 平脐蠕孢菌; 培养特性; 碳源; 氮源;
【Key words】 Bipolaris; culture characteristic; C-source; N-source;
【基金】 国家重点基础发展规划项目(G2000018602);国家自然科学基金项目(30471232)资助
  • 【文献出处】 草业学报 ,Acta Pratacultural Science , 编辑部邮箱 ,2005年05期
  • 【分类号】S54
  • 【被引频次】13
  • 【下载频次】179
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