【摘要】 以桑的子叶、下胚轴作为外植体,并将子叶等分为子叶尖部、子叶中部、子叶基部(靠近胚芽的一段),将下胚轴等分为下胚轴上段(靠近胚芽的一段)、中段、下段,置于培养基中培养。结果表明:子叶基部与下胚轴的上段离体再生效果好,子叶基部的离体再生成苗率可以达到63.5%,下胚轴上段的离体再生成苗率为47.1%。同时还发现分区子叶和分段下胚轴都是越接近胚芽生长点的部位用作外植体,其诱导出芽的时间短,并且能很好地分化再生成苗。更多还原

【Abstract】 Cotyledon and hypocotyle which were averagely parted 3 segments were cultured on MS solid medium supplemented with 3.0 mg/L 6-BA,0.3 mg/L IAA,1.0 mg/L AgNO₃.The result showed that plant regeneration rate of cotyledon and hypocotyle which are near the embryo was the highest. It reached 63.5% for cotyledon and 47.1% for hypocotyle. At the same time, the result showed that the cotyledon and the hypocotyle which are the nearer embryo,the shorter period is the inducement and the plant regeneration rate is the best.更多还原