【摘要】 根据家蚕微孢子虫rRNA基因高度保守的特点,设计合成了1对PCR引物,从家蚕微孢子虫基因组DNA上扩增出1个12kb片段,用同位素标记后作为检测家蚕微孢子虫的特异性探针。采用原位杂交的方法,对家蚕卵及感染家蚕微孢子虫4、6、10d的幼虫进行原位杂交检测。实验结果表明,该方法可以从家蚕单粒卵内检测到296粒孢子,对感染家蚕微孢子虫4、6、10d的幼虫均有阳性杂交信号出现。更多还原

【Abstract】 For the purpose of detecting Nosema bombycis by hybridization in situ, the probe(1.2 kb) was obtained by PCR from rRNA gene of Nosema bombycis and labeled by random primer synthesis technique with ~32 P-dATP.The result showed that at least 296 spores were detected from single silkworm egg,and the spores were also detected from the larvae infected by Nosema bombycis for 4 days ,6 days and 10 days respectively.更多还原