【摘要】 利用双向电泳技术分析了家蚕生态致死突变伴性赤蚁sch和正常黑蚁夏芳在常温常湿、高温干燥催青条件下的蚕卵差异表达蛋白质,发现高温敏感伴性致死赤蚁的蚕卵在高温催青处理后,一个分子量50kD左右的蛋白点P34与正常相比有明显差异,高温干燥处理后该蛋白点浓度较弱,表达量较低;对照黑蚁夏芳的P34蛋白没有明显差异。对P34进行胰蛋白酶消化并测定了其肽质量指纹图谱,通过同源性比较分析发现P34蛋白是一种新的蛋白质。更多还原

【Abstract】 In order to illustrate the mechanism of embryo lethality of sch,the proteins of sch silkworm egg incubated under normal condition and under high temperature and humidity are analyzed by two-dimensional gel electrophoresis.The result showed that the concentration of P3-4 protein of sch eggs incubated under different conditions is obviously different.The concentration of P3-4 of sch eggs incubated under high temperature and humidity was lower than that under normal condition.However,the control Xiafang have no difference under different conditions.Analyzed by matrix-assisted laser adsorption ionization time of flight mass spectrometry and searched silkworm database,the P3-4 may be a new protein.更多还原