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家蚕杂交分离系间的分子标记特征研究

The Character of Molecular Markers Between Segregation Lines of Hybridization in Silkworm Bombyx mori

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【作者】 司马杨虎陈大霞孙德斌何斯美钱荷英李斌鲁成向仲怀

【Author】 SIMA Yanghu~1,2 CHEN Daxia1 SUN Debin1 HE Simei3 QIAN Heying3 LI Bin1 LU Cheng~1* XIANG Zhonghuai1 (~1 The Key Sericultural Labortory of the Agricaltural Ministry; College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China; ~2 College of Agriculture Science and Technology, Soochow University, Suzhou 215006, China; ~3 The Sericultural Research Institute, Chinese Academy of Agriculture Sciences, Zhenjiang 212018, China)

【机构】 农业部蚕桑学重点开放实验室西南农业大学蚕学与生物技术学院中国农业科学院蚕业研究所西南农业大学蚕学与生物技术学院 重庆400716苏州大学农业科学与技术学院苏州215006重庆400716镇江212018重庆400716教授博士生导师

【摘要】 以两个日系家蚕品种“湘晖”(P1)和“872”(P2)为亲本进行杂交,从F1自交的F2代开始,分别向茧丝量高和低两个方向进行选择。经过F2-F7共6代的同蛾区交配选择,获得了茧丝量性状有显著差异的A、C两个家系。用5组引物扩增所获得的617个AFLP分子标记,分析了A、C两个杂交分离系的分子标记特征及其DNA多态性。结果显示P1能将更多的标记传给C系,亲本P2能将更多的标记传给A系,A系保留了更多的两亲共有的标记;A系含较稳定的特异性标记23个,C系含特异性标记7个,推测A系的这些特异性标记与家蚕某些经济性状有关;通过杂交分离系各世代的遗传距离和聚类分析,F6代同一家系内差异更小,A系与C系家系间的差异更加明显,即同蛾区交配到F6代系统就基本稳定。

【Abstract】 With the Japanese line “Xianghui”and “872” as the parents, the offspring after F2 was selected according to high (A line) or low (C line) cocoon silk amount respectively. Two strains A and C, which have significant differences in cocoon silk trait, were obtained by selection from F2 to F7. 617 molecular markers were obtained by AFLP from 5 primers. The molecular marker and DNA polymorphisms of two strains were analyzed. The results showed that C strain tends to like P1, while A strain tends to like P2, and that A strain has more common markers with parents. A strain had 23 specific markers, and C strain had 7 specific markers. The specific markers of A strain maybe relative to some economic quantitive traits of silkworm. The dendrogram by UPGMA suggested that it is less diversity among the individuals of F6 generation of same strain, and more diversity between different strain. Which suggested that the strain is stable in heredity while mating is within the same batch till F6 generation.

【关键词】 家蚕近亲交配选择分子标记
【Key words】 Bombyx moriSib mateGenetic breedingMolecular-marker
【基金】 国家863项目(编号2002AA211011);国家自然科学基金重点项目(编号30170719)。
  • 【文献出处】 蚕业科学 ,Acta Sericologica Sinica , 编辑部邮箱 ,2005年01期
  • 【分类号】S881.2
  • 【被引频次】11
  • 【下载频次】53
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