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硫酸铜,硝酸银和脱落酸对草地早熟禾胚性愈伤诱导的影响

Effect of CuSO₄,AgNO₃ and ABA on inducing of embryogenic callus of Kentucky Bluegrass

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【Author】 LUO Li~(1), HAN Lie-bao~(1),GAO Cai-xia~(2), Klaus K Nielsen~(2)( 1. Beijing Forestry University, Beijing 100083, China; 2. DLF Trifolium, research division, Store Heddinge 4660, Denmark)

【机构】北京林业大学草坪研究所； DLF Trifolium A/S； Store Heddinge； Store Heddinge 北京100083；北京100083； Denmark 4660； Denmark 4660；

【摘要】试验以草地早熟禾4个品种的成熟种子为外植体,研究了CuSO4、AgNO3、脱落酸(ABA)对草地早熟禾胚性愈伤诱导的影响,结果表明,K3诱导培养基中添加CuSO4对草地早熟禾胚性愈伤组织的形成有促进作用,除DP192-10之外的3个品种都发生了明显的形态上的变化,DP-LF-300和2W42-117在添加5μmol/LCuSO4的培养基上诱导出了乳白色、致密的胚性愈伤组织,2W42-117的胚性愈伤率高达40%。在MS基本培养基中添加AgNO3、ABA对草地早熟禾4个品种的胚性愈伤组织的诱导没有作用。更多还原

【Abstract】 The effect of CuSO₄,AgNO₃and ABA on embryogenic callus inducing to 4 varieties of Kentucky bluegrass （Poa pratensis L.） was investigated based on explant derived from mature seeds. The results showed that K3 induction medium supplemented with CuSO₄improved the formation of embryogenic callus. DP-LF-300 and 2W42-117 produced white to yellow, compact and friable embryogenic callus on K3 medium supplemented with 5 mol/L CuSO₄. High efficiency of embryogenic callus was obtained up to 40% in 2W42-117. While additionally supplemented AgNO₃and ABA in MS based medium showed ineffective on embryogenic callus formation.更多还原