【摘要】 将犊牛腹泻粪便经轮状病毒酶标诊断试剂盒检测阳性者,应用Marc145细胞培养,从河北省奶牛场分离出1株牛轮状病毒。分离毒株在Marc145细胞上产生明显的细胞病变,测其TCID50=10-4.70/0.1mL;提取该分离毒株的RNA,经聚丙烯酰胺凝胶电泳(PAGE)检测其电泳型为4∶2∶3∶2型,属于A群轮状病毒;RT-PCR方法扩增出342bp的目的条带,特异性检测均未见目的条带,进一步证实为轮状病毒。更多还原

【Abstract】 Absract :Astrain of bovine rotavirus identified by the diagnostic kit for rotavirus fromthe calveswith diarrhea of Hebei province was successfullyisolated and adaptedin Marc145 cells .It producedobvious cytopathic effects in Marc145 cells ,Its TCID50was 10-4.70/0.1 mL;Polyacrylamide gelelectrophoresis (PAGE) of its dsRNAgenome showed that its RNAelectropherotype was 4∶2∶3∶2which accorded with the electropherotype of group Arotavirus . The single expected size(342 bp)was produced by reverse transcription -polymerase chain reaction(RT-PCR) . The result showedthat a strain of bovine rotavirus was obtained.更多还原