【摘要】 通过对玉米大斑病菌RAPD反应程序中的一些重要参数进行摸索和优化试验,建立了一套适合玉米大斑病菌的扩增反应体系及反应程序:25μL体系中,加入Taq DNA聚合酶2 0 U、25 mmol L的MgCl2 2 0 mmol L、dNTP 200μmol L、随机引物30 ng、10×PCR buffer 1μL、DNA模板30 ng、用重蒸水补足25μL。反应程序为:94℃预变性3 min;94℃变性1 min, 37℃退火1 min,72℃延伸2 min,40循环; 72℃延伸6 min。更多还原

【Abstract】 The optimal RAPD reaction system of Setosphaeria turcica was established through experimentaion. In the total volume of 25 μL, there were 2 U Taq DNA polymerase, 2 mmol/L Mg²⁺, 200 μmol/L dNTP, 30 ng arbitrary primer, 1 μL 10×PCR buffer and 30 ng template DNA. Amplification was performed by the following program: 3 min at 94℃,40 cycles of 1min at 94℃, （1 min） at 37℃, 2 min at 72℃ and a final extention of 6 min at 72℃.更多还原