【摘要】 以花魔芋球茎切块作外植体,接种在 MS 附加6-BA、NAA 和 KT 的培养基上诱导愈伤组织的形成。各种培养基均能诱导外植体产生愈伤组织,但不同激素浓度组合其诱导率不一样。其中以 MS+6-BA 0.5mg/L+NAA 1.0mg/L 的诱导率最高,达84.62%。愈伤组织芽分化最适培养基是 MS+6-BA 1.0mg/L+NAA 0.5mg/L+KT2.0mg/L,分化率为73.81%。芽在 MS+NAA 0.5 nag/L 和1/2MS+NAA 0.5mg/L 培养基上的生根率均在78%以上。更多还原

【Abstract】 The corms of Amorphophallus rivieri Durieu,a species of the genus Amor- phophallus,were used as explants for tissue culture.The corms could be all induced to form callus when cultured on MS₁ medium supplemented with different hormone concen- trations,but the callus induction frequency was different,and the maximal frequency was 84.62% when cultured on MS+6-BA0. 5mg/L+NAA1.0mg/L.The callus was differ- entiated to bud on MS+6-BA1.0mg/L+NAA0. 5mg/L+KT2.0mg/L with differen- tial rate being 73.81%.The rootage rate was both above 78% on the medium MS+ NAA0.5mg/L and 1/2MS+NAA0.5mg/L更多还原