【摘要】 目的:对细胞系中支原体的感染情况进行检测并分型。方法:利用支原体 16S~23S保守区域扩增来检出细胞系中支原体感染率,并利用 16S~23S保守区间的间隔区(spacer)长度不同进行巢式PCR扩增,结合测序达到分型的目的。结果:在检测的 22株细胞系中,有 77. 3% (17株)的细胞系有支原体感染,其中 5株有 2种或 2种以上支原体感染,在分布上以M.fermentans和M.hyorhinis支原体多见。结论:支原体在细胞系中的高感染率提示,在以细胞系作为模型的研究中,对支原体感染所造成的影响应有充分认识。检测及分型技术的建立为进一步研究不同型别支原体的致病力提供了可能。更多还原

【Abstract】 Objective:To detect the rate of Mycoplasma infection in cell lines and further determine its types. Methods:We performed nest PCR amplification of Mycoplasma’s conserved regions (16S-23S) and sequenced the spacer with different length between conserved regions. Results:Within the tested 22 cell lines, 17 (77.3%) showed Mycoplasma infections, of which 5 had two or more types of Mycoplasma. M.fermentans and hyorhinis were more frequently detected within the types of infected Mycoplasma. Conclusion:The high rate of Mycoplasma infection in cell lines makes it necessary for researchers to pay more attention to its influence on research data when using cell lines as models. Establishment of detection and classifying techniques make it possible to further study the pathogenesis of different types of Mycoplasma.更多还原