【摘要】 目的 :建立同步多色荧光原位杂交技术在外周血、羊水及单个胚胎细胞及骨髓细胞等样本中的实验方法 ,探索其在细胞遗传学、产前诊断、胚胎种植前诊断以及在血液病中的应用。方法 :取外周血淋巴细胞、羊水细胞和不适于胚胎移植及冷藏的受精胚胎细胞、骨髓细胞分别制片 ,用相应的荧光标记的探针进行原位杂交 ,荧光显微镜取图分析。结果 :建立获得稳定可行的各类标本的实验方法。结论 :应用多色荧光原位杂交技术可对染色体进行快速准确的诊断 ,并可应用于产前诊断和胚胎种植前诊断及血液病的诊断。更多还原

【Abstract】 Objective: To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples. Methods: FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene. Results: FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations. Conclusion: Multi-color FISH is clinically useful tool, which can be used as an adjunct to conventional chromosome analysis for prenatal diagnosis , preimplantation genetic diagnosis and diagnosis of leukemia.更多还原