【摘要】 目的:探讨局部脑缺血对大鼠下丘脑的促肾上腺皮质激素释放激素(CRH)和神经生长因子(NGF)以及下丘脑-垂体-肾上腺轴(HPA)的影响。方法:选用正常老龄Wistar大鼠32只,雌雄各半,随机分组,每组4只大鼠。分假手术组和实验组,实验组分为缺血15 min组、缺血15 min再灌注1 h、6 h、1 d、2 d4、d、9 d组。采用免疫组化方法对老龄大鼠局部脑缺血下丘脑的CRH和NGF表达进行动态观察。结果:假手术组下丘脑有少量CRH和NGF表达,实验组在缺血再灌注1 h内下丘脑CRH和NGF表达均无变化,此后缺血再灌注6 h下丘脑CRH和NGF均有短暂一过性消失,随后缺血再灌注2 d二者在下丘脑的表达相继明显增加。结论:脑缺血后下丘脑CRH分泌细胞并非立刻进入活动增强状态;脑缺血后下丘脑后续阶段存在CRH分泌细胞的活动增强效应;NGF对维系HPA的活动可能具有重要作用。更多还原

【Abstract】 Objective To investigates the influences of focal cerebral ischemia on CRH,NGF and HPA in hypothalamus of rats.Methods Thirty-two senile Wistar rats including fourteen female and fourteen male rats were divided into seven groups at random and there were four rats per group.One group was control group,the other six groups were operation groups.Operation groups consisted of ischemia 15 min group and ischemia 15 min groups in which rats were reperfused for 1,6 h and 1,2,4,9 d.The expressions of CRH and NGF in hypothalamus of focal cerebral ischemia senile rats were observed dynamically with the immunohistochemical method.Results There was a little expressions of CRH and NGF in hypothalamus in control group,the expressions of CRH and NGF in hypothalamus didn′t change during 1 h after ischemia reperfusion in operation groups,then the expressions of CRH and NGF in hypothalamus disappeared transiently at 6 h after ischemia reperfusion,but after that the expressions of CRH and NGF in hypothalamus increased obviously at 2 d after ischemia reperfusion.Conclusion The activity of CRH secretory cells in hypothalamus is not immediately in the strength phase after cerebral ischemia;the activity strength effect of CRH secretory cells in hypothalamus exists in the later stage after cerebral ischemia;NGF is possibly important to the activity of HPA.更多还原