【摘要】 目的:探讨胰岛素样生长因子- (IGF- )在局灶性脑缺血再灌注损伤中的表达及作用。方法:采用线栓法制备大鼠局灶性脑缺血模型,应用免疫组化染色、原位杂交技术检测IGF- 蛋白及m RNA表达。结果:正常组织有极低水平的IGF- 表达,脑缺血后表达主要位于半影区,随缺血及缺血再灌注时间延长,半影区表达逐渐增强,IGF- 蛋白及m RNA表达在再灌注2 4 h为16 9.6 4±6 .97、16 8.6 4±6 .2 8,4 8h为16 8.86±5 .39、170 .86±5 .0 7,并达高峰。结论:内源性IGF- 表达增强,对局灶性脑缺血再灌注损伤具有保护作用。更多还原

【Abstract】 Objective To study the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) in focal cerebral ischemical reperfusion injury model and its contribution. Methods The focal cerebral ischemia rat models were induced using the filament method, the expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA was dectected by the method of immunohistochemical staining and in situ hybridization technique. Results The expression of IGF-Ⅰ was very low in normal tissues, while increased mainly in penumbra after cerebral ischemia. The expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA were 169.64±6.97 and 168.64±6.28,respectively, at 24 h after reperfusion and peaked to 168.86±5.39 and 170.86±5.07 at 48 h after reperfusion. Conclusion Enhanced expression of endogenous IGF-Ⅰ has protective effects on focal cerebral ischemia reperfusion injury.更多还原