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嘧肽霉素影响烟草花叶病毒RNA蓄积量的研究

Influence of Cytosinpeptidemycin on the Accumulation of Tobacco Mosaic Virus RNAin Tobacco Leaves

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【作者】 吴元华； 朱春玉； 杜春梅； 王艳红； 赵秀香；

【Author】 WU Yuan-hua~1, ZHU Chun-yu~1, DU Chun-mei~2, WANG Yan-hong~1, ZHAO Xiu-xiang~1(1.College of Plant Protection, Shenyang Agricultural University, Shenyang110161, China; 2.College of Life Sciences of Heilongjiang University,Haerbin150080, China)

【机构】 沈阳农业大学植保学院； 黑龙江大学生命科学学院； 沈阳农业大学植保学院 沈阳110161； 沈阳110161； 哈尔滨150080； 沈阳110161；

【Abstract】 A new method, quantitative competitive PCR with software of Image Master ED, was used for quantitative detection of TMV-RNA in infected plant tissues and those treated with 4% Cytosinpeptidemycin. Cytosinpeptidemycin is a newly reported antiphytoviral agricultural antibiotics. Three different treatments were adopted in this study. The results indicated that all treatments reduced TMV-RNA accumulation in TMV infected tobacco leaves treated with 4% Cytosinpeptidemycin. These treatment of mixture of Cytosinpeptidemycin and TMV at the equal volume reduced TMV-RNA accumulation greatly. The results were also confirmed by indirect ELISA technique. In conclusion, Cytosinpeptidemycin inhibited the infectivity of virus by reducing RNA accumulation in infected plants.

【关键词】 竞争PCR； 嘧肽霉素； 烟草花叶病毒； RNA；
【Key words】 <Keyword>Competitive PCR； Cytosinpeptidemycin； TMV；