【摘要】 目的:探讨依托泊苷(VP 16 )单独或联合端粒酶11 聚体寡核苷酸对肺腺癌细胞的作用。方法:VP 16单独或联合脂质体介导的端粒酶11 聚体寡核苷酸与肺腺癌细胞株共培养,MTT法检测细胞生长抑制率;流式细胞术检测细胞周期及细胞凋亡;以TRAP ELISA法半定量测定细胞端粒酶活性。结果:VP 16抑制肺腺癌细胞株SPC A1细胞生长,抑制率18.9% ,下调端粒酶活性,与未加VP 16比较均有明显不同(P <0 .0 1) ,可诱导细胞凋亡。VP 16联合反义寡核苷酸(ASODN)与联合正义寡核苷酸(SODN)对SPC A1细胞的抑制率分别为4 3.5 %和4 1.0 % ,两者差异无显著性(P >0 .0 5 ) ;联合用药效果明显优于单用VP 16 ,差异有显著性(P <0 .0 1)。结论:化疗药联合短链正、反义核苷酸可抑制肺癌细胞生长,较二者单独应用可提高疗效,同时降低副作用更多还原

【Abstract】 Objective:To investigate the effect of etoposide(VP-16),in combination with oligodeoxynucleotides on lung adenocarcinoma cells.Methods:Etoposide in combination with telomerase 11-base oligodeoxynucleotides werer transfected into SPC-A 1 cell line by lipofection.The multiparameters such as cell growth inhibition rate,cell cycle and apoptasis rate,and telomerase activity were detected by MTT,FCM,and PCR-ELISA,respectively.Results:Etoposide inhibited cell growth(with an inhibition rate of 18%),decreased telomerase activity and induced cell apoptosis.ASODN+VP-16 and SODN+VP-16 inhibit the growth of SPC-A 1 cell line and rate are 43.5% and 41.0%,repectively(P>0.05).The difference was significant when compared with VP-16(P<(0.01)).Conclusions:Chemotherapeutant in combination with short chain oligodeoxynucleotides is more effective than used alone in inhibiting lung cancer and the side effect can be greatly reduced.更多还原