【摘要】 采用盐桥支撑技术制备镶嵌胆固醇的双分子层卵磷脂(脑磷脂)膜,用以模拟细胞膜。用正辛烷做溶剂,对不同卵磷脂(脑磷脂)与胆固醇配比进行制膜,膜的稳定性均超过100h。实验发现,纯脂质体不能输运重金属离子,胆固醇能输运重金属离子。胆固醇与重金属离子之间存在吸附作用,无化学反应。采用电化学工作站对双分子层脂膜的稳定性、离子通透性、双层膜中胆固醇对离子通透性的影响等进行了研究,并利用实验制备的双分子层脂膜模拟研究锌离子跨细胞膜的输运过程,建立了锌离子跨细胞膜的吸附-扩散模型,该模型计算值与实验值基本吻合。更多还原

【Abstract】 This paper aims to introduce the authors’ simulation of the cell on the bilayer lipid （phosphatidylcholine or cephalin） membranes （BLM） inlaid with cholesterol supported by salt bridge. To this end, we have prepared a great variety of such membranes （whose stability is to exceed 100 hours） by using the octane as solvent, a different ratio of phosphatidyl choline or cephalin to the cholesterol as solute. Our experimental results show that pure lipid can not transfer heavy metal ions while the cholesterol can. The experiments also show that the adsorption really exists between cholesterol and heavy metal ions, and not chemical reaction. The paper has also made a study of the stability of bilayer lipid membranes, the membrane permeation of ions, and the effects of cholesterol on the membrane permeation of ions by means of electrochemical analytical devices. Thus, we have established an adsorption-diffusion simulation model for Zn²⁺ transfer across BLM and calculation of its transfer in conformity with the experimental results.更多还原