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优化黄姜中甾体皂甙元测定的研究
Improvement on Determination of Diosgenin in Dioscorea zingiberensis Wright
【摘要】 对传统的黄姜中甾体皂甙元的测定方法进行了改进。提取甾体皂甙元时采用微量样品以氯仿浸提代替传统的回 流抽提法,以Liebemann-Barcharad试剂显色,用分光光度法测定。测定结果准确,高效低成本且步骤简单,应用广泛。
【Abstract】 Improvement was made on the traditional way of determination of diosgenin. Diosgenin was extractied with chloroform instead of circumfluence and then oxidized by Liebemann-Barcharad reagent and finally determined with a spectrophotometer. This method was proved to be simple, accurate, low-cost and highly efficient.
- 【文献出处】 氨基酸和生物资源 ,Amino Acids & Biotic Resources , 编辑部邮箱 ,2005年01期
- 【分类号】TQ464
- 【被引频次】9
- 【下载频次】170