【摘要】 背景与目的:鼻咽癌(nasopharyngealcarcinoma,NPC)致病的分子机制至今仍不清楚,已有研究表明,染色体3p21～22区域存在与鼻咽癌发生密切相关的抑癌基因。KIAA1173基因是定位于3p22.1的一个新的肿瘤相关基因,其与NPC发病的关系尚未见报道。本研究采用KIAA1173基因特异性原位杂交探针,检测其在NPC组织及细胞株中的表达,探讨KIAA1173基因与NPC发病的关系。方法:克隆KIAA1173基因片段(354bp),并制备cDNA探针;采用组织芯片技术,通过原位杂交检测73例鼻咽部不同组织标本(41例NPC、18例鼻咽非典型增生上皮、14例正常鼻咽粘膜上皮)和6种NPC细胞株(CNE1、CNE2、HNE1、HNE2、6-10B、5-8F)中KIAA1173基因mRNA的表达情况。结果:KIAA1173基因在NPC细胞、非典型增生上皮和正常鼻咽粘膜上皮的阳性率分别为21.9%(9/41)、83.3%(15/18)、92.8%(13/14),而6种NPC细胞株均未见表达;在正常鼻咽粘膜上皮和非典型增生上皮中强阳性率分别是64.3%(9/14)和38.9%(7/18),而NPC中无强阳性,在鼻咽部不同上皮组织中的表达差异有显著性(P<0.001);在38例伴有淋巴细胞浸润的NPC组织中,癌细胞与浸润淋巴细胞之间KIAA1173基因表达有显著性差异(P=0.026),并且呈明显负相关(κ=-0.337,P=0.020)。结论:KIAA1173基因在鼻咽部不同组织中表达不同,正常鼻咽上皮强表达,而NPC细胞中低表达甚至不表达,提示该基因可能参与NPC演变的过程。更多还原

【Abstract】 BACKGROUND & OBJECTIVE: Although the molecular etiology of nasopharyngeal carcinoma (NPC) is still unknown, studies showed that there are NPC-associated tumor suppressor genes residing in chromosome 3p21-22. KIAA1173 gene, locates at 3p22.1, was characterized as a new carcinoma-related gene, while its correlation to tumorigenesis of NPC hasn’t been reported yet. This study was to detect the expression of KIAA1173 gene in NPC tissues and cell lines, and investigate its involvement in NPC. METHODS: KIAA1173 gene fragment (354 bp) was cloned, and the cDNA probe was prepared. The expression of KIAA1173 gene in 73 nasopharyngeal tissue samples (including 41 specimens of NPC, 18 atypical hyperplasia epithelia, and 14 normal nasopharyngeal mucosa epithelia) and 6 NPC cell lines (including CNE1, CNE2, HNE1, HNE2, 6-10B, and 5-8F) were examined using tissue microarray technique by in situ hybridization (ISH). RESULTS: The positive rates of KIAA1173 mRNA were 21.9% (9/41) in NPC, 83.3% (15/18) in atypical hyperplasia epithelia, 92.8% (13/14) in normal nasopharyngeal mucosa epithelia, and 0 in all NPC cell lines. Its strongly positive rate was significantly lower in NPC than in atypical hyperplasia epithelia and normal mucosa epithelia (0 vs. 38.9% and 64.3%, P<0.001). In 38 specimens of NPC with infiltrated lymphocytes, the positive rate of KIAA1173 mRNA was significantly lower in cancer cells than in tumor infiltrating lymphocytes (23.7% vs. 44.7%, P<0.05); the expression of KIAA1173 in cancer cells was negatively related to that in tumor infiltrating lymphocytes (κ=-0.337, P<0.05). CONCLUSIONS: KIAA1173 gene is strongly expressed in normal nasopharyngeal mucosa epithelia, but down-regulated in NPC. It may be associated with the tumorigenesis of NPC.更多还原