【摘要】 为寻求KCHIP1基因与乳腺癌之间的关系,对收集到的12例乳腺癌组织、12例正常乳腺组织样本进行KCHIP1基因cDNA扩增、突变检测。经测序证实,在这些乳腺癌组织样本中没有发现KCHIP1基因的突变,但发现了KCHIP1基因一种新的剪接型,这个新剪接型是在原来KCHIP1基因外显子1和外显子2之间多了一个162bp的插入片段(AY780424)。更多还原

【Abstract】 The present study aimed to find the mutations of KCHIP1 gene in breast cancer. KCHIP1 cDNA samples from 12 specimens of breast cancer and 12 specimens of normal mammary tissues were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and directly sequenced to detect mutation. No mutation of KCHIP1 gene was found in these samples; while a new splicing type of KCHIP1 gene was found, which has an insert (162 bp) between exon 1 and exon 2 in KCHIP1 gene (AY780424).更多还原