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HPLC法测定白芍不同饮片中芍药苷的含量

Determination of paeoniflorin of different processed products of radix paeoniae alba by HPLC

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【作者】 张家富孟楣邢安之

【Author】 ZHANG Jia-fu,MENG Mei,XING An-zhi(The First Hospital to Anhui Collage of TCM,Hefei230031,China)

【机构】 安徽中医学院第一附属医院药学部安徽中医学院第一附属医院药学部 安徽合肥230031安徽合肥230031安徽合肥230031

【摘要】 目的比较加工炮制对白芍不同饮片中芍药苷含量的影响。方法采用高效液相色谱法,色谱柱:Nova-PakC18,流动相:甲醇-水溶液(30∶70);流速:0.8ml.min-1;检测波长:230nm,柱温为25℃。结果各样品中,以生白芍中芍药苷含量为高(4.12%);各炮制品中以麸炒白芍中芍药苷含量为高(2.67%)。加样回收率为99.49%,RSD为0.46%,相关系数r=0.9996。结论白芍临床以麸炒制品入药为宜。

【Abstract】 AimTo compare the effect of different processing methods on the content of paeoniflorin in radix paeoniae alba.MethodsThe HPLC method was used for the determination.Chromatographic condition includes:Nova-pak C18column,methanol-water(3070)as a mobile phase,UV detector wa-velength at 230 nm,25 column temperature and 0.8 mlmin-1 of flow rate.ResultsThe content of paeoniflor in of the sheng-baishaopian was the highest among all samples.The content of paeoniflorin of the fuchao-baoshao was the highest among all processed products.Its recovery was 99.49% and RSD was 0.46%.The correlation coefficient was 0.9996.ConclusionIt is better to use radix paeoniae alba in the form of the fuchao-baishao in clinic.

【关键词】 HPLC白芍炮制芍药苷
【Key words】 <Keyword>HPLCradix paeoniae albaprocessingpaeoniflorin
  • 【文献出处】 安徽医药 ,Anhui Medical and Pharmaceutical Journal , 编辑部邮箱 ,2005年08期
  • 【分类号】R284.1
  • 【被引频次】50
  • 【下载频次】772
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