【摘要】 目的对尿液中各种蛋白质进行分离,用于区分尿蛋白的类型。方法应用十二烷基磺酸钠-琼脂糖凝胶电泳(SDS-AGE)法进行非浓缩尿蛋白电泳,对40例已被临床确诊的肾脏疾病患者的尿液样本进行电泳分析,使尿蛋白按分子量大小进行分离。结果根据尿蛋白分子量大小可以将其区分为肾小球性、肾小管性、混合性、溢出性及生理性蛋白尿。其中肾小球性蛋白尿13例、肾小管性蛋白尿9例、混合性蛋白尿11例、溢出性蛋白尿2例、生理性蛋白尿5例。结论本法具有分离效果好,检测灵敏度和诊断符合率高,操作简便,速度快,胶片易于保存等优点。可以将尿蛋白按分子量大小进行区分,经EDC扫描后可获半定量结果,对肾脏疾病的受损部位和受损程度的判断具有较高的价值。更多还原

【Abstract】 Objective Separate various kinds of urine protein to discriminate the type of each one.Methods By undertook unconcentrated urine protein electrophoresis with method of sodium dodecyl sulphate-agarose gel electrophoresis (SDS-AGE),we analyzed 20 urine samples selected from 20 clinical definited patients suffering kidney disease and separate urine proteins according to molecular weight.Results We can discriminate glomerular proteinuria,tubuloproteinuria,mixed albuminuria,overflow proteinuria and physiologic proteinuria according to molecular weight of urine protein.Cuj.Cujus 13 of them were glomerular proteinuria,9 tubuloproteinuria,11 mixed albuminuria,2 overflow proteinuria and 5 physiologic proteinuria.Conclusion SDS-AGE have the merits of outstanding discrimination results,high sensitivity and diagnose accordance rate,convenient manipulation,fast velocity,easy conservation with film.Urine protein can be discriminated according to molecular weight,and semiquantitative results can be obtained after scanning,so it is valuable in estimating damaged part and damaging degree in the kidney.[更多还原