【摘要】 运用组织培养技术对鹅掌楸穴LiriodendronchinenseSarg.雪的种子及冬芽进行诱导,通过初代培养、继代培养、生根培养及炼苗等技术研究,筛选出最适宜的培养基配方。结果表明:冬芽诱导的最佳配方为WPM+穴0.2～2.0雪mg/LBAP鸦种子发芽诱导最佳配方为MS+0.05mg/LBAP鸦继代培养最佳配方为MS+0.2mg/LBAP+0.05mg/LNAA或WPM+0.2mg/LBAP;生根培养最佳配方为WPM或1/2WPM+穴1.0～2.0雪mg/LNAA,1/2MS+0.2mg/LIBA+0.1mg/LNAA+0.1mg/LABT;炼苗最佳基质为蛭石。更多还原

【Abstract】 The seeds and buds of Chinese tulip tree as explants were cultured to research the optimal media for mass propagation. The appropriate treatments for buds and seeds were WPA+(0.2～2.0)mg/L BAP or MS + 0.05 mg/L BAP. The suitable media for subculture was MS + 0.2 mg/L BAP + 0.05 mg/L NAA or WPM + 0.2 mg/L BAP, and the appropriate media for rooting inducement was WPM(1/2WPM) + (1.0～2.0)mg/L NAA or 1/2MS + 0.2mg/L IBA + 0.1 mg/L NAA + 0.1 mg/L ABT. Vermiculite was the preferable material for plantlet growth.更多还原