【摘要】 研究了不同取材时间、不同基本培养基和不同浓度的 6 -BA和NAA组合对沙棘组培快繁的影响。结果表明 :以春秋季节休眠芽为外植体获得的沙棘组培苗效果最好 ;B5+ 6 -BA 0 .5mg/L +NAA 0 .5～ 1.0mg/L为适宜的启动培养基 ,对休眠芽的诱导率为 10 0 % ;1/2B5+ 6- BA 0 .5mg/L +NAA 0 .5mg/L为最佳继代增殖培养基 ;1/2B5+ 6 -BA 0 .1mg/L +NAA 1.0mg/L为最佳生根培养基 ,生根率达到 85 % ;试管苗经过驯化移栽成活率达 5 0 %以上。更多还原

【Abstract】 In this article,the influence of several factors on micropropagation of Hippophae rhamnoides was investigated.The results showed: B₅ medium containing 0.5mg/L 6-BA and 0.5～1.0mg/L NAA was suitable for initial culture; the highest rate for shoot propagation were obtained on 1/2B₅ medium supplemented with the same concentration of 6-BA（0.5mg/L） and a lower concentration of NAA（0.5mg/L）;1/2B₅ medium supplemented with 0.1mg/L 6-BA and 1.0mg/L NAA were found to be the best medium for root initiation and growth; after in vitro hardening, the plantlets were transplanted to soil where the plants grew well.更多还原