【摘要】 背景与目的:研究热休克蛋白47基因(Heat shock protein 47,HSP47)在小鼠胚胎腭、前肢正常和异常的发育过程中的表达情况。材料与方法:在GD10经口一次分别给予各实验组孕鼠80mg/kg的全反式视黄酸,对照组孕鼠给予等体积的植物油,并分别于GD11~GD18取两组胎鼠的前肢,于GD15~GD17取两组胎鼠的腭,利用RT_PCR方法半定量检测HSP47的表达丰度。结果:HSP47在所有样品中均有表达,其在GD11~GD18正常、异常发育肢的表达均呈随胚龄增大而增加的趋势,对照肢和实验肢分别于GD16、GD17达到高峰,以后基本恒定;其在GD15~GD17正常腭中恒定表达、异常腭中以GD16表达最高;异常肢的表达丰度在GD11~GD18均高于正常肢,正常腭和异常腭的表达丰度在GD15无差异,但在GD16~GD17异常腭的表达丰度则高于正常腭。结论:全反式视黄酸所致的短肢和腭裂中,HSP47的表达呈应激性升高。更多还原

【Abstract】 BACKGROUND&AIM: To study the expression of heat shock protein47(HSP47)in normal forelimbs and short forelimb malformations,normal palates and cleft palates during mouse embryogenesis. MATERIAL AND METHODS: At GD10,gestational mice of the treatment and the control groups were administered with80mg/kg all-trans retinoic acid and the same volume vegetable oil separately.The forelimbs of embryos were harvested in GD11-GD18,and the normal palates and cleft palates were obtained in GD15-GD17.The relative abundance of HSP47of all samples was measured by reverse transcript polymerase chain reaction(RT-PCR). RESULTS: HSP47was expressed in all samples.To the normal forelimbs and short forelimb malformations,with the embryos aging,there was the increasing tendency of the expressional abundance of HSP47in GD11-GD18,their peaks of expression were especially at GD16and GD17,and kept stable in the following age.The expressional abundance of HSP47of the normal palates was stable in GD15-GD17,and the peak of that of the cleft palates was at GD16.The expression of HSP47of the abnormal limbs was stronger than that of the normal limbs in GD11-GD18.The expression of HSP47of the cleft palates has no difference with that of the normal palates in GD15,but it was stronger than that of the cleft palates in GD16-GD17. CONLUSION: The expressional abundance of HSP47in normal limbs and short limb malformations,normal palates and cleft palates induced by all-trans retinoic acid during mouse embryogenesis was increased by stress action.更多还原