【摘要】 目的　观察胰岛素对脑缺血再灌注损伤的治疗作用 ,并探讨其作用机制。方法　用线栓法建立大鼠脑缺血再灌注模型 ,根据胰岛素的给药时间不同共分成 2大组 ,第 1大组再分为A、B、C、D 4组 ,第 2大组再分为 2组。在第 1大组检测各组不同时限的血糖 ,测量计算脑梗死灶体积 ,并进行神经功能缺损评分 ;在第 2大组采用TUNEL法原位标记DNA片段 ,检测TUNEL阳性细胞的变化。结果　在 6h内给予胰岛素治疗可使神经功能缺陷评分显著降低 ,脑梗死灶体积缩小 ,TUNEL阳性细胞也明显减少 (P <0 .0 5 )。结论　早期应用胰岛素能减轻脑缺血再灌注损伤 ,减轻再灌注后细胞损伤可能是其作用机制之一。更多还原

【Abstract】 Objective To study the protection of insulin on focal cerebral ischemia-reperfusion injury.Methods We establish the model of cerebral ischemia-reperfusion in rats with suture-occluded method. The rats were divided into 2 big groups according to the different injection time of insulin. In first big group which was divided into A, B, C, D subgroups, the blood glucose levels, scores of neurological deficit and infarct volume were estimated. At the same time the terminal deoxynucleotiolyl transfer erase-mediated dUTP-biotin nick end labeling DNA fragments in situ(TUNEL) were used to detect the change of the TUNEL positive cells in the second big groups.Results The scores of neurological deficit and infarct volume were lower significantly in the treated groups. The numbers of the TUNEL positive neuron were also fewer in the treated groups than control groups. Conclusion Insulin can reduce cerebral ischemia-reperfusion injury and it should be used within effective treatment time window (6 h).更多还原