【摘要】 目的研究脑梗死后脑组织的物质和能量代谢的变化及丹参在脑梗死治疗中的作用。方法 18只 SD 大鼠随机分成梗死组、丹参组和假手术组3组,梗死组和丹参组用光化学法诱导颞叶皮层梗死.假手术组仅手术暴露颞骨。丹参组于诱导梗死前10min 腹腔注射丹参注射液5g/kg,于24、48h 按此剂量各再给药1次,假手术组和梗死组大鼠腹腔注射生理盐水;然后运用磁共振成像和波谱技术,观察脑梗死后1、3、5、7天磁共振 T2加权像和氢磷谱的动态变化以及丹参的作用。结果假手术组大鼠颞叶皮层的 T2加权像信号正常,梗死组大鼠颞叶皮层出现一高信号区。于梗死后3、5、7天,梗死组大鼠该区的 NAA/Cho 加 Cr 与假手术组比较,差异均有显著性(P<0.05);假手术组的 Lac 一直处于检测水平线以下,而梗死组高信号区的 Lac 于梗死后1、3、5、7天均可检测到。于梗死后1、3和5天,梗死组大鼠全脑的βATP/PME 加 PDE 和 PCr/PME 加PDE 与假手术组比较,差异均有显著性(P<0.05);于梗死后1天,梗死组大鼠全脑的βATP/PCr 与假手术组比较,差异有显著性(P<0.05)。而丹参能减小梗死大鼠梗死后1、3、5和7天 T2加权像上的高信号区的大小(P<0.05)及5和7天该区与对侧相应区域的信号强度比(P<0.05),能减少梗死后5和7天该区的 NAA/Cho 加 Cr 的下降(P<0.05)以及 Lac/Cho 加 Cr(P<0.05)和 Lac/NAA(P<0.05)的增高,能减少梗死后1天全脑的βATP/PME 加 PDE 和βATP/PCr 的下降(P<0.05)。结论脑梗死后梗死区脑组织出现水肿,乳酸升高,以及神经元功能受损和死亡,全脑出现能量代谢障碍;丹参能有效地减轻这些异常变化。更多还原

【Abstract】 Objective To study changes of substance and energy metabolism of brain tissue after cerebral infarction,and the function of Radix Salviae miltiorrhizea in treating cerebral infarction.Methods By means of T2 weighted magnetic reso- nance image（MRI）,¹H and ³¹P-magnetic resonance spectroscopy （MRS）,and photochemically induced temporal cortical infarction rat model,dynamic changes of T2 weighted MRI,¹H and ³¹P-MRS were observed on the 1st,3r d,5th and 7th day after cerebral infarction,and also observed the effect of Radix Salviae mihiorrhizea.Results T2 signal on temporal lobe cortex was normal in rats of the sham-operation group.High signal region was found temporal lobe cortex in rats of in- farction group.The volume and signal intensity of the region were gradually decreased as time passed by.NAA/Cho+Cr of that region was lower than that in the corresponding region in sham-operation group on the 1st day,and gradually de- creased on the 3rd and 5th day,showing significant difference on the 3th,5th and 7th day（P<0.05）.Lac appeared in the high signal region of the rats in the infarction group 1 day after infarction,and obviously increased again within 5 days,and still remained higher level on the 7th day,while Lac in the corresponding region of rats in the sham-operation group remained below the tested level.βATP/PME+PDE,PCr/PME+PDE and βATP/PCr of rats in the infarction group were lower than those in the sham-operation group 1 day after infarction,and gradually increased after that.Compared with the sham-operation group,there was significantly difference in βATP/PME+PDE and PCr/PME+PDE of 1,3,5 day after infarction,and β ATP/PCr of 1 day after infarction （P<0.05）.Radix Salviae miltiorrhizea could lower the high signal re- gion area of T2 weighted image 1,3,5,and 7 days after infarction,and lower the signal strength ratio of that area and the contralateral corresponding region 5 and 7 days.It also could lower the decrement of NAA/Cho+Cr and the increment of Lac/Cho+Cr and Lac/NAA of that region 5 and 7 days after infarction,and could lower the decrement of β ATP/PME+ PDE and β ATP/PCr 1 day after infarction.Conclusion:Edema,neuron function injury and death appeared in infarction area after infarction,increased lactic acid,energy metabolism disturbance appeared in the whole brain.Radix Salviae mil- tiorrhizea could effectively lessen these abnormal changes.更多还原