【摘要】 目的观察参附注射液对控制性失血性休克兔肝、小肠、胰腺再灌注损伤的保护效应。方法 18只健康家兔随机分为假手术组,对照组和治疗组,(每组6只)。采用 Lamson 休克模型,维持平均动脉压(MAP)在40mmHg 90min,再灌注90min 后检测肝、小肠、胰腺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、肿瘤坏死因子(TNFα)及细胞间黏附分子1(ICAM-1)水平。观察对照组和治疗组的小肠末段组织超微结构。结果再灌注90min 后治疗组兔肝、小肠、胰腺 MDA、TNFα及 ICAM-I 水平显著低于对照组(P<0.05或P<0.01);SOD 水平显著高于对照组(P<0.05)。结论参附注射液可在一定程度上减少兔失血性休克后腹腔脏器再灌注损伤的脂质过氧化反应和炎性反应。更多还原

【Abstract】 Objective To observe the protective effect of Shenfu Injection (SFI) on ischemia/reperfusion (I/R) injury of liver,small intestine and pancreas in rabbits with controlled hemorrhagic shock.Methods Eighteen healthy rabbits were randomly divided into the sham operation group,the control group and the treated group,six in each.Using Lam- son shock model,after mean arterial pressure (MAP) being maintained at 40 mmHg for 90 rain,followed with 90 min of reperfusion,levels of malondialdehyde (MDA),superoxide dismutase (SOD),tumor necrosis factor α(TNF-α) and intercellular adhesion molecule-1 (ICAM-1) in liver,small intestine and pancreas tissue were measured.And the ultra- structure of terminal segment of small intestine was observed.Results After 90 min of reperfusion,levels of MDA, TNF-α and ICAM-1 in rabbits’ liver,small intestine and pancreas were lower and level of SOD was higher in the treat- ed group than those in the control group (P<0.01 or P<0.05) respectively.Conclusion SFI could reduce the lipid peroxidation and inflammatory reaction to certain extent in celiac viscera of hemorrhagic shock rabbits after I/R in- jury.更多还原